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Sysmex CyFlow Cube 8

Sysmex CyFlow Cube 8 image

Sysmex CyFlow Cube 8

High performance flow cytometry

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High performance flow cytometry

  • Configurations with up 8 optical parameters (up to 6 colours)
  • Choice of lasers with 375, 407, 488, 532, 561, 594, 638, 640, 785 nm
  • Particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE)
  • Optional CyFlow Robby 8 Autoloading Station

High-performance flow cytometry

The CyFlow Cube 8 is a compact flow cytometer for the analysis of single cells and microscopic particles with a high grade of integration. The CyFlow Cube 8 offers the unique combination of a truly stand-alone system of a small footprint with a modular configuration concept with up to 6 colours. The easy-to-use CyFlow software provides control of the instrument, data acquisition, analysis and storage. The possibility of customised instrument settings facilitates switching between different applications. CyFlow Cube 8 offers True Volumetric Absolute Counting (TVAC), which allows the display of particle concentrations for any subsets of cells without the need for reference beads, even if defined by a gate at a later time after the acquisition.

* Optional CyFlow Robby 8 Autoloading Station for well plates and tubes
* Optional CyFlow Sorter for closed, non-destructive cell and particle sorting with high purity

CyFlow: Intuitive software for CyFlow Cube 8

The newly designed, intuitive CyFlow acquisition and analysis software offers the measurement of millions of cells at your fingertips.

  • Quickly choose your plots for analysis from all of the combinations shown in Preview Mode.
  • Easily compare plots with the Overlay Wizard, for single or dual parameters.
  • Share and customise your results within your research organisation with the Report Generator.
  • Change your view with Zoom and V-Log Scale; you are even able to gate in Zoom Mode.
  • Compensate rapidly with the Compensation Wizard.
  • CyFlow optimally supports the True Volumetric Absolute Counting of the CyFlow Cube 8, displaying particle concentrations for any subsets of cells, even if defined by a gate at a later time after the acquisition.

Size / weight

500 x 470 x 355 mm

 

The principle of operation

Fluorescent flow cytometry

Wavelength
  • blue laser - 488 nm
  • red laser - 635 nm
 
Specifications

Light source and optics

  • Optical parameters: 8 (FSC, SSC, FITC, PE, PerCP-Cy5.5, PE-Cy7, APC, APC-Cy7)
  • Light sources: 2 lasers (488 nm, 50 mV and 640 nm, 40 mV)

Flow system

  • Fluorescence sensitivity: <100 MESF (FITC), <50 MESF (PE)
  • Fluorescence resolution: CV <2
  • Detection particle size: 0.1–100 nm
  • Automatic absolute case counting (TVAC) and syringe-controlled volume control

Electronics and signal processing

  • Parallel signal processing from each optical channel
  • Linear or logarithmic - to choose from
  • 16-bit analog-to-digital converters, with the ability to change the detection parameter and curve level
  • Number of detected cases: up to 15,000 cases / sec